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Protein Biochemistry
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Calcein-AM
Calcein-AM from BioLegend
Be the first to write a review!
Citations:
(21)
Supplier Page
Supplier Page from
BioLegend for
Calcein-AM
Description
Calcein-AM
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Calcein-AM
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
425201
Quantity
10 x 50 µg
Applications
Flow Cytometry (FC)
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (21)
(1)
A human mesenchymal spheroid prototype to replace moderate severity animal procedures in leukaemia drug testing
F1000Research
November 6, 2023
Aaron Wilson, Sean Hockney, Jessica Parker, Sharon Angel, Helen Blair, Deepali Pal
Dorset, UK",GAAGATGGTGATGGGATTTC,OLIGO
Staining
Reagents,
Staining
Reagents,
Calcein AM
,
BioLegend
,
425201
Hoechst 33342,"MERCK, Sigma Aldrich", PBS,
Gibco
,15400-054 FITC
CD105
Antibody,
BioLegend
,
323203
AlexaFluor
(2)
3D-printed scaffold harboring copper ions combined with near-infrared irradiation for local therapy of cancer
iScience
October 20, 2023
Hao Wei, Dong Chen, Bin Han, Peng Li, Hao Jia, Lizhang Zhu, Hao Yang, Deren Lan, Wei Wei, Haibo Chen,
et al.
and dead
cell staining
, cells or
organoids
were double-stained in the dark with 5 μM of
calcein-AM
(
425201
,
Biolegend
, USA) and 5 μM of propidium iodide (PI) (
421301
,
Biolegend
) for 20 min. Then, the cells or
(3)
Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligera
Frontiers in Cell and Developmental Biology
October 11, 2023
Tal Gordon, Noam Hendin, Omri Wurtzel
(
Thermo Fisher
,
H3570
) and
DRAQ5
(
Abcam
,
ab108410
), to a final concentration of 20 μM.
Calcein-AM
(
BioLegend
, #
425201
) and
Calcein Violet-AM
(
BioLegend
, #
425203
) were used as live
cell labeling
dye by supplementing to
(4)
Truncated ring-A amaryllidaceae alkaloid modulates the host cell integrated stress response, exhibiting antiviral activity to HSV-1 and SARSCoV-2
Scientific Reports
January 30, 2023
James McNulty, Chanti Babu-Dokuburra, Jon Scattolon, Carlos Zepeda-Velazquez, Maribeth A. Wesesky, Jill K. Caldwell, Wenxiao Zheng, Jadranka Milosevic, Paul R. Kinchington, David C. Bloom,
et al.
h.
Organoids
viability
Organoids
viability was analyzing using
calcein AM
assay
(
BioLegend
; catalogue #
425201
), according to manufacturer’s instructions. Briefly,
organoids
were exposed singularly in Eppendorf
(5)
Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection
Viruses
January 27, 2023
Abeynaike SA, Huynh TR, Mehmood A, Kim T, Frank K, Gao K, Zalfa C, Gandarilla A, Shultz L, Paust S.
Tissue Analysis Software. 2.6. Calcein Release
Assay
K562 cells
were stained with
Calcein-AM
(Cat#
425201
,
BioLegend
, San Diego, CA, USA) and incubated with PBMCs derived from
human
peripheral blood or splenocytes
(6)
Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq
STAR Protocols
December 16, 2022
Ãlvaro Quintanal-Villalonga, Joseph M. Chan, Ignas Masilionis, Vianne Ran Gao, Yubin Xie, Viola Allaj, Andrew Chow, John T. Poirier, Dana Pe’er, Charles M. Rudin,
et al.
(
red blood cell
lysis buffer),
Lonza
,#
10-548E
Human
TruStain FcX,
BioLegend
,#
422302
Calcein AM
,
BioLegend
,#
425201
"
DAPI (4,6-Diamidino-2-Phenylindole Dihydrochloride
)",
Invitrogen
,#
D1306
Ficoll Paque PLUS
,
GE Healthcare
,#
17144003
-500
(7)
The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function
eLife
October 3, 2022
Li S, Bern MD, Miao B, Fan C, Xing X, Inoue T, Piersma SJ, Wang T, Colonna M, Kurosaki T,
et al
.
protein",
Recombinant mouse IL-18
,
MBL
,Catalog#
B002-5
, "Chemical compound, drug",
Calcein-AM
,
Biolegend
,Catalog#
425201
, "Software, algorithm",Prism 9,GraphPad,https://www.graphpad.com/, "Software, algorithm",FlowJo 10,Treestar,https://www.flowjo.com/,
(8)
Biomaterial-Mediated Protein Expression Induced by Peptide-mRNA Nanoparticles Embedded in Lyophilized Collagen Scaffolds
Pharmaceutics
August 2, 2022
Oude Egberink R, Zegelaar HM, El Boujnouni N, Versteeg EMM, Daamen WF, Brock R.
Then, 500 µL of 3 µM calcein-acetoxymethyl ester (
Calcein-AM
,
BioLegend
, San Diego, CA, USA, Cat No.
425201
) and 2 µg mL−1 of propidium iodide (PI,
Sigma-Aldrich
, Cat No.
P4170
) in PBS were added to the scaffolds
(9)
Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification
Advanced Science
August 1, 2022
Derick Yongabi et al.
the harvested cells for live imaging were resuspended in DPBS containing 2 µm
Calcein‐AM
(
Biolegend
,
425201
), 2 µg mL−1 Alexa Fluor 594
wheat
germ agglutinin (
Thermo Fisher Scientific
,
W11262
), 1 µm Hoechst 33342
(10)
Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4
Journal of cellular and molecular medicine
April 1, 2022
Yang F, Liu XQ, He JZ, Xian SP, Yang PF, Mai ZY, Li M, Liu Y, Zhang XD.
seeded on Matrigel and incubated at 37°C for 6–8 h. The cells were then stained with 1 μM
Calcein‐AM
(
425201
,
Biolegend
, San Diego, CA, USA) for 30 min and imaged with a
fluorescence microscope
(Nikon Corporation,
(11)
A Novel Anti-B7-H3 × Anti-CD3 Bispecific Antibody with Potent Antitumor Activity
Life (Basel)
January 21, 2022
Yan Feng et al.
USA). 2.6.
In Vitro Cytotoxicity Assay
Target cells (5 × 103–1 × 104) labeled with 2 μM
calcein-AM
(
425201
,
BioLegend
, San Diego, CA, USA) were co-cultured with effector cells and the indicated concentrations
(12)
Surfaceome Profiling of Rhabdomyosarcoma Reveals B7-H3 as a Mediator of Immune Evasion
Cancers (Basel)
September 9, 2021
Roxane R. Lavoie et al.
B7-H3KO cells were plated in a 96-well plate and incubated 30 min at 37 °C with
calcein-AM
(
Biolegend
,
425201
). The cells were then washed twice with
Live Cell Imaging Solution
(
Gibco
,
A14291DJ
) and kept in RPMI-1640
(13)
ADAM17 Inhibition Increases the Impact of Cisplatin Treatment in Ovarian Cancer Spheroids
Cancers (Basel)
April 23, 2021
Nina Hedemann et al.
with 2× final concentration of the following dyes in the medium were added.
Calcein-AM
(
BioLegend
, #
425201
), final concentration of 0.1 µg/mL, was used to stain living cells; propidium iodide (PI) (
BioLegend
,
(14)
KIR3DL3 Is an Inhibitory Receptor for HHLA2 that Mediates an Alternative Immunoinhibitory Pathway to PD1
Cancer Immunology Research
February 1, 2021
Rupal S. Bhatt et al.
transfected
Raji cells (both β2-microglobulin
knockout
) were stained with 1 μM
calcein AM
(
BioLegend
; cat. #
425201
) in PBS for 20 minutes at room temperature then washed twice with PBS and resuspended in RPMI1640 medium
(15)
Serial single-cell profiling analysis of metastatic TNBC during Nab-paclitaxel and pembrolizumab treatment
Breast Cancer Research and Treatment
January 1, 2021
Jiehui Deng et al.
BioLegend
) and single cells were obtained and stained with
DAPI
(Cat #
422801
,
Biolegend
) and Calcein (Cat #
425201
,
Biolegend
). The BD Aria III sorter was used with a 100 μm nozzle to sort live (DAPI- Calcein+) single
(16)
Natural display of nuclear-encoded RNA on the cell surface and its impact on cell interaction
Genome biology
September 10, 2020
Huang N, Fan X, Zaleta-Rivera K, Nguyen TC, Zhou J, Luo Y, Gao J, Fang RH, Yan Z, Chen ZB,
et al
.
following the manufacturer’s protocol. Cells were stained with
Calcein AM
(2.5 μM,
Biolegend
cat. #
425201
) for 15 min at 37 °C for live-cell fluorescent tracking. Meanwhile, oligonucleotide probes were denatured
(17)
Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA
Science advances
July 1, 2020
Wang K, Lin RZ, Hong X, Ng AH, Lee CN, Neumeyer J, Wang G, Wang X, Ma M, Pu WT,
et al
.
with h-iEC media. After 6 hours, cells were incubated with 1 μM
Calcein-AM
(
BioLegend
, catalog no.
425201
) for 10 min and then imaged using a
fluorescence microscope
. Numbers of branches were counted by ImageJ. In
(18)
Analyzing the Quenchable Iron Pool in Murine Macrophages by Flow Cytometry
BIO-PROTOCOL
March 20, 2020
Michael Riedelberger, Karl Kuchler
8-hydroxyquinoline (
Sigma-Aldrich
, catalog number: H6878) 28.
Calcein-AM
(
BioLegend
, catalog number:
425201
) 29.
Dimethyl sulfoxide (DMSO
) (
Sigma-Aldrich
, catalog number:
D2438
) 30. Trypsin solution 10x (Sigma-Aldrich,
(19)
Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation
Stem Cells International
October 15, 2019
Guoyi Dong et al.
measure
the cell viability, hMSC spheroids at days 1, 3, and 6 were stained with
Calcein-AM
(
BioLegend
,
425201
) and PI (
Sigma
,
P4170
-25MG) according to the manufacturer’s protocol. Briefly, spheroids were incubated
(20)
Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies
mAbs
May 1, 2019
Trinklein ND, Pham D, Schellenberger U, Buelow B, Boudreau A, Choudhry P, Clarke SC, Dang K, Harris KE, Iyer S,
et al
.
the
co-culture
assay
. Target cell lines were fluorescently labeled with 5 μM
Calcein AM
(
Biolegend
#
425201
) and incubated in with 2.5 mM probenecid (
Sigma
#
P8761
). Each
biological assay
was set up in 96-well
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Calcein-AM from BioLegend
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View Company Product Page
Pricing Info
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